A kinetic study on the enzymatic hydrolysis of fluorescein diacetate and fluorescein-di-beta-D-galactopyranoside |
| |
| Authors: | J Hofmann M Sernetz |
| |
| Institution: | Institut für Biochemie und Endokrinologie, Fachbereich Veterinärmedizin und Tierzucht, Justus-Liebig-Universität Giessen, Frankfurter Strasse 100, D-6300, Giessen, Federal Republic of Germany |
| |
| Abstract: | The kinetics of the hydrolysis of fluoresceindiacetate and fluorescein-di-beta-D-galactopyranoside were investigated by thin-layer chromatography. The time course of the concentrations of substrate, monosubstituted intermediate, and product was simulated numerically. The mathematical model takes into account the competition of substrate and intermediate and the accumulation of the intermediate at the enzyme. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
