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Genomic characterization of ϕRS603, a filamentous bacteriophage that is infectious to the phytopathogen Ralstonia solanacearum
Authors:Truong Thi Bich Van  Shohei Yoshida  Kaito Miki  Akihiro Kondo  Kaeko Kamei
Institution:1. Department of Biomolecular Engineering, Kyoto Institute of Technology, , Sakyo‐ku, Kyoto, 606‐8585;2. Hiyoshi, , Omihachiman, Shiga, 523‐8555 Japan
Abstract:
A filamentous bacteriophage (?), ?RS603, which is infectious to the phytopathogen Ralstonia solanacearum was isolated. ?RS603 was found to have a circular single‐stranded DNA genome composed of 7679 nucleotides and to contain 13 putative open reading frames (ORFs). The ?RS603 genome showed strong similarity with those of Ralstonia phages ?RSM1 and ?RSM3, as reported by Askora et al. The ?RS603 genome had no ORFs corresponding to ORFs 2, 3, 13 and 14 (integrase) of ?RSM3. ?RS603 had an ORF that was homologous to other Ralstonia phages ?RSS0 and ?RSS1; however, ?RSM1 and ?RSM3 did not.
Keywords:Bacteriophage  genome  Ralstonia solanacearum
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