Sequential assignment of the 1H and 31P resonances of the double stranded deoxynucleotide d (ATGCAT)2 by 2D-NMR correlation spectroscopy |
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Authors: | D Marion G Lancelot |
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Affiliation: | Centre de Biophysique Moléculaire (C.N.R.S.) 1A, Avenue de la Recherche Scientifique 45045 Orléans Cedex, France |
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Abstract: | 31p-1H and 1H-1H chemical shift correlation spectroscopy are jointly used for providing a complete assignment of sugar proton (except H5' and H5") and phosphorus resonances in the double stranded oligonucleotide d (ATGCAT)2. In contrast to previous methods the specific assignment of overcrowded H5' H5" proton resonances is not required. Using the H3'-P coupling and also the long range H4'-P coupling, this quite general method can be easily implemented on intermediate field spectrometer. The present results pave the way to the 1H and 31P resonance assignment of longer double-stranded oligonucleotides. |
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Keywords: | 2D-NMR Two-dimensional nuclear magnetic resonance COSY correlated spectroscopy DSS 2-2 dimethyl-2-silapentane-5-sulfonate D.Q.F. double quantum filter nOe Nuclear Overhauser effect |
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