Identification and quantitation by radioimmunoassay of prostaglandin F compounds in bile

Separation and identification of prostaglandins in canine bile was performed by extraction and thin layer chromatography. The system provided tentative identification of the prostaglandin F compounds as the major prostaglandin subgroup present in bile. The prostaglandin was subsequently purified on silicic acid columns and quantitated by radioimmunoassay with tritiated PGF_2α and anti PGF_2α antibody employing the double antibody technique. Basal levels in hepatic bile were found to be 1028 ± 98 pg/ml.