Quantification of binding of some thiol-reactive clenbuterol analogues to bovine serum albumin by electron paramagnetic resonance spectroscopy |
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Authors: | Prezelj Andrej Strancar Janez Gubensek Franc Pecar Slavko Degand Guy |
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Affiliation: | Faculty of Pharmacy, University of Ljubljana, Ljubljana 1000, Slovenia. abdrej.prezelj@ffa.uni-lj.si |
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Abstract: | The only free thiol group of bovine serum albumin (BSA) was coupled in a high yield with some novel thiol-reactive clenbuterol analogues. The unreacted cysteines were probed with maleimide spin label to determine the yield of the coupling reaction. A novel approach to determining free thiol groups of BSA quantitatively by electron paramagnetic resonance spectroscopy and spectral decomposition without the usual gel-filtration step or extensive dialysis is presented. |
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Keywords: | BSA Clenbuterol Electron paramagnetic resonance Thiol spin labeling |
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