HPLC法测定葛花中鸢尾苷的含量

目的：测定8个产地葛花中鸢尾苷的含量,建立葛花中鸢尾苷含量测定的HPLC方法。方法：采用GRACEC18（250mm×4.6mm,5μm）色谱柱,流动相为乙腈-水梯度洗脱,流速为0.8mL/min,紫外检测波长为265nm;柱温为室温。结果：鸢尾苷的峰面积（Y）与浓度（X）在11.8～236.4μg/mL范围内具有良好线性关系,Y=34920X-1156.5,r=0.9995（n=7）;平均加样回收率为103.66%,RSD〈2%（n=9）;测得8批不同产地的葛花药材中鸢尾苷含量在37.00～113.1mg/g。结论：建立了高效液相色谱法测定葛花中鸢尾苷含量的方法,该法准确、可靠,可用于葛花中主要成分鸢尾苷的含量测定;不同产地葛花中均检测到鸢尾苷,但其含量有一定区别。

Determination of Tectoridin in Flos Puerariae by HPLC

Objective：To develop an HPLC method for determination of tectoridin in Flos Puerariae, and to provide valuable data for quality control of Flos Puerariae. Methods： The samples were separated on a GRACE C18 （250 mm ×4.6 mm,5 μm） column with the mobile phase of acetonitrile -water （gradient elution）. Flow rate was 0.8 mL/min. The detection wavelength was set at 265 nm. Column temperature was ambient temperature. Results： The linear range of tectoridin was 11.8 - 236.4μg/mL, and the linear function was Y = 34920X - 1156.5 （ r = 0. 999 5 ）. The average recovery was 103.66% , and RSDs were less than 2% （ n = 9）. The contents of tectoridin in Flos Puerariae were 37.00 - 113.1 mg/g. Conclusion： The method for quantitation of tectoridin in Flos Puerariae was accurate and reliable, which can be used to evaluate the quality of Flos Puerariae.