Towards a saturated sorghum map using RFLP and AFLP markers |
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| Authors: | K Boivin M Deu J-F Rami G Trouche P Hamon |
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| Institution: | (1) CIRAD-CA, BP 5035, F-34032 Montpellier cedex, France, FR;(2) RUSTICA PROGRAIN GENETIQUE, 7 rue hermès, Parc technologique du canal, F-31520 Ramonville St-Agne, France, FR;(3) CIRAD/INERA, 01 BP596, Ougadougou, Burkina Faso, BF;(4) Université P Valery, route de Mende, F-34199 Montpellier cedex 5, France/CIRAD, BP 5035, Montpellier cedex, France, FR |
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| Abstract: | A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite,
essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes
343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes,
it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from
12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different
data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be
noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is
induced after adding the AFLPs.
Received: 10 May 1998 / Accepted: 13 July 1998 |
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| Keywords: | Sorghum RFLP AFLP Genetic linkage map |
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