Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of<Emphasis Type="Italic">Aspergillus oryzae</Emphasis> |
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Authors: | Joo-Hyung?Heo Vladimir?Ananin Jeong-Seok?Park Chung-Ryul?Lee Jun-Ok?Moon Ohsuk?Kwon Hyun-Ah?Kang Chul?Ho?Kim Email author" target="_blank">Sang?Ki?RheeEmail author |
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Institution: | (1) Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, 305-600 Daejeon, Korea;(2) BioHoldings Inc., BVC #201, Korea Research Institute of Bioscience and Biotechnology, 305-600 Daejeon, Korea |
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Abstract: | The influences of impeller types on morphology and protein expression were investigated in a submerged culture ofAspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells
that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation
grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production
for both the intracellular heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase).
The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation
ofA. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted
in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller
agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged
fed-batch fermentation of recombinantA. oryzae. |
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Keywords: | Aspergillus oryzae filamentous fungi morphology propeller agitation submerged culture fed-batch fermentation |
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