Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags |
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Authors: | J?D?Jung H-W?Park Y?Hahn C-G?Hur D?S?In H-J?Chung J?R?Liu Email author" target="_blank">D-W?ChoiEmail author |
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Institution: | (1) Eugentech Inc., 52-Oun Dong, Yusong, 305–333 Daejon, Korea;(2) Korea Research Institute of Biosciences and Biotechnology, 52-Oun Dong, Yusong, 305–333 Daejon, Korea |
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Abstract: | Expressed sequence tags (ESTs) provide a valuable tool that can be used to identify genes in secondary metabolite biosynthesis. Ginseng (Panax ginseng C.A Meyer) is a medicinal plant that accumulates ginsenosides in roots. We sequenced 11,636 ESTs from five ginseng libraries in order to create a gene resource for biosynthesis of ginsenosides, which are thought to be the major active component in roots. Only 59% of the ginseng ESTs exhibited significant homology to previously known polypeptide sequences. Stress- and pathogen-response proteins were most abundant in 4-year-old ginseng roots. ESTs involved in ginsenoside biosynthesis were identified by a keyword search of BLASTX results and a domain search of ginseng ESTs. We identified 4 oxidosqualene cyclase candidates involved in the cyclization reaction of 2,3-oxidosqualene, 9 nine cytochrome P450 and 12 glycosyltransferse candidates, which may be involved in modification of the triterpene backbone.Abbreviations cDNA Complementary DNA - ESTs Expressed sequence tagsCommunicated by I.S. Chung |
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Keywords: | Expressed sequence tags Ginsenoside Panax ginseng Oxidosqualene cyclase Secondary metabolism |
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