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A novel microRNA miR-1165-3p as a potential diagnostic biomarker for allergic asthma |
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| Authors: | Chaojie Wu Kun Xu Zhengxia Wang Zhongqi Chen Zhixiao Sun Wenqing Yu |
| Affiliation: | 1. Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China;2. Department of Respiratory Medicine, The Affiliated Wuxi No. 2 People’s Hospital of Nanjing Medical University, Wuxi, China;3. Department of Infectious Disease, Taizhou people's Hospital, Taizhou, China |
| Abstract: | Context: A further examination of a novel miRNA,miR-1165-3p as a biomarker for asthma, which was previously implicated in helper T cells (Th2) in a murine asthma model. Objective: To determine whether serum miR-1165-3p can serve as a potential diagnostic biomarker for allergic asthma. Methods: Serum miR-1165-3p was quantified via quantitative real-time PCR (qRT-PCR) in asthmatic and control samples. Serum miR-1165-3p levels were compared between groups and the clinical diagnostic abilities of miR-1165-3p were evaluated. The analyses utilized included a student’s t test, one-way ANOVA, and the generation of receiver operating characteristic (ROC) curves. Results: Serum miRNA-1165-3p levels were significantly elevated in asthmatics when compared to the healthy controls. Furthermore, the sensitivity and specificity of serum miR-1165-3p were found to be 83% and 68.2%. Additionally, serum miR-1165-3p levels were also found to be significantly elevated in patients with allergic rhinitis (AR) or allergic bronchopulmonary aspergillosis (ABPA). Conclusions: This study showed that serum miR-1165-3p can potentially be utilized as a noninvasive biomarker that is able to aid in the diagnosis and characterization of allergic asthma. |
| Keywords: | Asthma miR-1165-3p allergic rhinitis (AR) allergic bronchopulmonary aspergillums (ABPA) biomarker Th2 |