Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile <Emphasis Type="Italic">Bacillus coagulans</Emphasis> |
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Authors: | Kenneth M Bischoff Siqing Liu Stephen R Hughes Joseph O Rich |
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Institution: | (1) U.S. Department of Agriculture, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA |
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Abstract: | A strain of Bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to l-lactic acid with 85% yield at 50°C was isolated from composted dairy manure. The strain was tolerant to aldehyde growth inhibitors
at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. In a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate
of 100 g corn fiber/l to 39 g lactic acid/l in 72 h at 50°C. Because of its inhibitor tolerance and ability to fully utilize
pentose sugars, this strain has potential to be developed as a biocatalyst for the conversion of agricultural residues into
valuable chemicals. |
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