rDNA targeted oligonucleotide primers for the identification of pathogenic yeasts in a polymerase chain reaction |
| |
Authors: | Jack W Fell |
| |
Institution: | (1) Rosenstiel School of Marine and Atmospheric Science, University of Miami, 4600 Rickenbacker Causeway, 33149 Key Biscayne, Florida, USA |
| |
Abstract: | Summary Species-specific oligonucleotide primers were designed for PCR identification of the basidiomycetous yeastsCryptococcus neoformans, Trichosporon cutaneum andRhodotorula mucilaginosa. The procedure uses standard PCR components including DNA from the test species and three primers: two universal external (upstream and downstream) limiting primers and a species-specific internal primer. Species identification requires the formation of a species-specific rDNA nucleotide segment that is significantly smaller (200 bp) than a non-target segment (600 bp). The procedure can be used to identify yeasts from single and mixed populations.This paper is dedicated to Professor Herman Jan Phaff in honor of his 50 years of active research which still continues. |
| |
Keywords: | Cryptococcus neoformans Rhodotorula mucilaginosa Trichosporon cutaneum Basidiomycetes Molecular taxonomy Polymerase chain reaction |
本文献已被 SpringerLink 等数据库收录! |