A newly-isolated marine methanogen harbors a small cryptic plasmid |
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| Authors: | Alvin G. Wood William B. Whitman Jordan Konisky |
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| Affiliation: | (1) Department of Microbiology, University of Illinois, 131 Burrill Hall, 407 S. Goodwin, 61801 Urbana, IL, USA;(2) Department of Microbiology, University of Georgia, 30602 Athens, GA, USA |
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| Abstract: | Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a singleAval site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci. |
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| Keywords: | Methanogenic bacteria Plasmid isolation Alkaline lysis CsCl gradient Restriction endonuclease mapping Electron microscopy DNA homology |
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