Mu-1 directed inhibition of DNA breakdown in Escherichia coli, recA cells. |
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| Authors: | F. Van Vliet M. Couturier J. De Lafonteyne E. Jedlicki |
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| Affiliation: | (1) Laboratoire de Génétique, Faculté des Sciences, Université libre de Bruxelles, Belgique;(2) Unité de Virologie INSERM U102, Laboratoire de Génétique moléculaire, Lille, France;(3) Present address: Department of Histology and Genetics, Rijksuniversiteit Gent, Belgium;(4) Institut Pasteur de Lille, Boulevard Louis XIV, F-59012 Lille Cedex, France |
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| Abstract: | Summary The spontaneous DNA breakdown exhibited by recA strains, is reduced after heat induction of a thermoinducible Mu-1 prophage. This inhibition is dependent upon RNA synthesis, suggesting that Mu-1 directs synthesis of a recBC nuclease inhibitor, analogous to the product of the  gam gene. The genetic evidence presented here shows that Mu-1 enables a red gam phage to grow on a recA host. The in vitro assay for ATP-dependent exonuclease activity reveals a complete inhibition of this activity 30 min after induction of the Mu-1 prophage. |
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