DNA structures recognized by the human UV-DDB protein. |
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| Authors: | T Mizukoshi Y Fujiwara S Iwai |
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| Institution: | Department of Bioorganic Chemistry, Biomolecular Engineering Research Institute, Suita, Osaka, Japan. |
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| Abstract: | DNA recognition by the human UV-damaged DNA-binding (UV-DDB) protein was characterized. By circular permutation analyses, DNA duplexes containing the (6-4) photoproduct and the abasic site analog were found to be bent at angles of 54 degrees and 57 degrees, respectively, when they formed a complex with this protein. Although kinked NMR structures have been reported, fluorescence resonance energy transfer experiments revealed that these duplexes had no intrinsic bend. These results suggest that the UV-DDB protein binds DNA that can be bent easily at the above angle. |
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