Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins |
| |
Authors: | Percipalle Piergiorgio Jonsson Andreas Nashchekin Dmitri Karlsson Christina Bergman Tomas Guialis Apostolia Daneholt Bertil |
| |
Institution: | Department of Cell and Molecular Biology, Karolinska Institutet, Box 285, SE-171 77 Stockholm, Sweden. |
| |
Abstract: | Pre-mRNP complexes were isolated from rat liver nuclei as 40S hnRNP particles, and actin-binding proteins were collected by DNase I affinity chromatography. The bound proteins were analyzed by 2D gel electrophoresis, and the following five hnRNP A/B-type proteins were identified by tandem mass spectrometry: DBP40/CBF-A (CArG binding factor A), a minor hnRNP A2 variant and three minor hnRNP A3 (mBx) variants. DBP40 was chosen for further analysis of the association of actin with the pre-mRNP complex. It was shown in vitro that purified actin binds to recombinant DBP40 suggesting that the interaction between actin and DBP40 is direct in the pre-mRNP particles. The association of actin with DBP40 was further explored in vivo. It was shown in a transfection study that DBP40 appears both in the nucleus and cytoplasm. Microinjection experiments revealed that DBP40 is exported from the nucleus to the cytoplasm. Finally, RNA–protein and protein–protein cross-linking experiments showed that DBP40 interacts with poly(A)+ RNA as well as actin, both in the nucleus and cytoplasm. We propose that actin associated with DBP40, and perhaps with additional hnRNP A/B-type proteins, is transferred from nucleus to cytoplasm bound to mRNA. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|