Deletion Mutant Library for Investigation of Functional Outputs of Cyclic Diguanylate Metabolism in Pseudomonas aeruginosa PA14 |
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| Authors: | Dae-Gon Ha Megan E Richman George A O'Toole |
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| Institution: | aGeisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, Hanover, New Hampshire, USA ;bClaremont McKenna College, Keck Science Department, Claremont, California, USA |
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| Abstract: | We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library. |
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