Ornithine carbamoyltransferase of Streptomyces fradiae: purification and properties |
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Authors: | Istvá n Szilá gyi,Gyö rgy Vargha,Gá bor Szabó |
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Affiliation: | Institute of Biology, University Medical School Debrecen, Hungary |
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Abstract: | Abstract The enzyme ornithine carbamoyltransferase was purified from Streptomyces fradiae . A 1200-fold increase in specific activity was achieved by ammonium sulphate precipitation, DEAE-cellulose and aminohexyl-agarose chromatography and gel filtration. The purified enzyme has a M r of 87 000. Its isoelectric point is 5.3 as determined by isoelectric focusing. Apparent K m values at pH 7.7 for ornithine and carbamoyl phosphate are 1.8 and 1.2 mM, respectively. |
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Keywords: | Streptomyces fradiae Ornithine carbamoyltransferase (Purification) |
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