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High-density DNA probe arrays for identification of staphylococci to the species level
Authors:Couzinet Sabine  Jay Corinne  Barras Christine  Vachon René  Vernet Guy  Ninet Béatrice  Jan Isabelle  Minazio Marie-Antoinette  Francois Patrice  Lew Daniel  Troesch Alain  Schrenzel Jacques
Affiliation:Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli-du-Crest 24,1211 Geneva 14, Switzerland.
Abstract:Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.
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