Assignment of YAC Clones Spanning Rice Chromosomes 10 and 12 |
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| Authors: | Shimokawa Takanori; Kurata Nori; Wu Jianzhong; Umehara Yosuke; Ashikawa Ikuo; Sasaki Takuji |
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| Institution: | 1Rice Genome Research Program, Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 305 Japan
2National Institute of Agrobiological Resources 2-1-2 Kannondai, Tsukuba, 305 Japan |
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| Abstract: | Yeast artificial chromosome (YAC) clones were assigned on rice(Oryza sativa L. cv. Nipponbare) chromosomes 10 and 12 usingDNA markers from our high-density linkage map. Out of 1,383markers localized in this genetic map, 68 and 74 markers werelocated on chromosomes 10 and 12, respectively. Screening ofthe YAC genomic library was conducted by colony hybridizationand Southern hybridization using restriction fragment lengthpolymorphism (RFLP) markers or by polymerase chain reaction(PCR) using sequence-tagged site (STS) markers. We have completedthe screening of 68 markers on chromosome 10 and 74 markerson chromosome 12. A total of 134 and 103 YACs were assignedto chromosomes 10 and 12, respectively, with an estimated coverageof more than 60% for chromosome 10 and about 47% for chromosome12. As rice is considered a model plant for genome analysis,the ordered YAC clones on chromosomes 10 and 12 as well as otherchromosomes will certainly be helpful for isolation of agronomicallyand biologically important genes and for understanding the genomestructure of these chromosomes. |
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| Keywords: | rice DNA marker genome yeast artificial chromosome minimum overlap |
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