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Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus |
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| Authors: | Kosma, Paul Wugeditsch, Thomas Christian, Rudolf Zayni, Sonja Messner, Paul |
| Affiliation: | Zentrum für Ultrastukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie 1Institut für Chemie, Universität für Bodenkulter Gregor-Mendel-Stra$$$e 33, A-1180 Wien 2Scientific Software Comp. A-1140 Wien, Austria |
| Abstract: | The characterization of the S-layer glycoprotein of Bacillusihermoaerophilus revealed unexpected novelties. The isolationand purification procedure had to be changed due to completesolubility in aqueous buffers of the constituting S-layer protomers.Upon degradation of the S-layer glycoprotein by pronase andpurification of the products by gel filtration, ion-exchangechromatography, chromatofocusing and HPLC, one representativeglycopeptide fraction was selected for further characterization.From the combined evidence of composition analysis, chemicaldegradation, NMR spectroscopy experiments and comparison withsynthesized model substance, we propose the following repeatingunit structure of the glycan chain: |
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