Antioxidant capacity of desferrioxamine and ferrioxamine in the chemically-initiated lipid peroxidation of rat erythrocyte ghost membranes |
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Authors: | L A Videla T Cáceres E A Lissi |
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Institution: | Departamento de Ciencias Biológicas, Facultad de Medicina, Universidad de Chile, Santiago. |
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Abstract: | 2,2'-Azo-bis-(2-amidinopropane) induces the thermal lipid peroxidation of red blood cells membranes by a mechanism that is not iron dependent. The peroxidation rate, as assessed by oxygen uptake or visible chemiluminescence measurements, can be diminished by micromolar concentrations of desferrioxamine (DF), with a median inhibitory concentration (the concentration of DF that reduces the lipid peroxidation rate to 50% of that observed without scavengers addition) of 10 microM. In these conditions, the DF/Fe3+ (1:2) complex is nearly five times less efficient than DF. The present data show that DF is able to trap the initiator radicals and/or the free radicals involved in the lipid peroxidative chain at micromolar concentrations, range in which the agent cannot be used as a general test for iron involvement. |
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