Effect of tobacco smoke components on organogenesis in plant tissues |
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Authors: | KOCHHAR, T. S. BHALLA, P. R. SABHARWAL, P. S. |
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Affiliation: | Department of Botany, University of Kentucky Lexington Kentucky 40506, U.S.A. |
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Abstract: | Callus tissue derived from haploid plants of Nicotiana tabacumdifferentiated when inoculated on media fortified with differentconcentrations of tobacco smoke components: benzo(a)pyrene (BaP),benzo(e)pyrene (BeP), dibenz(a, h)anthracene (DBA), pyrene andchrysene. Neither any auxins nor cytokinins were present inthe medium. BaP was found to be the most potent morphogeneticagent. However, these treatments did not induce any vegetativebuds on the callus obtained from diploid plants of tobacco. 1 This study was carried out under Contract No. 12-14-100-10341(73)with the Agricultural Research Service, U. S. Department ofAgriculture, administered by the Eastern Marketing and NutritionResearch Division, 600 East Mermaid Lane, Philadelphia, Pennsylvania19118, U.S.A. (Received February 4, 1971; ) |
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