Role ofS gene product of bacteriophage lambda in host cell lysis |
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Authors: | S Barik N C Mandal |
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Institution: | (1) Department of Biochemistry, Bose Institute, 93/1, Acharya rafulla Chandra Road, 700 009 Calcutta;(2) Present address: Department of Microbiology, University of Connecticut Health Center, 06032 Farmington, Connecticut, USA |
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Abstract: | Studies with the induced lysogens of λS
+R+, λS-R+, λS+R- and λS-R- phages have shown that while theS gene product is essential for the action of intracellularR gene product to release the periplasmic alkaline phosphatase in the presence of EDTA, the latter gene product can bring about
this effect while acting onEscherichia
coli cells from outside, in the absence of functionalS gene product; chloroform, could help the intracellularR gene product in effecting bacterial lysis in the absence ofS gene product. These result support the premise that theS gene product facilitates theR gene product in crossing the cytoplasmic membrane into the periplasmic space such that the latter can act on the peptidoglycan
layer of the host cell thus causing both the release of alkaline phosphatase and cell lysis.
An erratum to this article is available at . |
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Keywords: | Bacteriophage λ λ S andR genes periplasmic leakage host cell lysis |
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