A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions. |
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Authors: | Millicent Masters John B. March I. R. Oliver J. F. Collins |
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Affiliation: | (1) Department of Molecular Biology, University of Edinburgh, King's Buildings, EH9 3JR Edinburgh, UK |
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Abstract: | Summary The sequence of the PcnB protein of Escherichia coli, a protein required for copy number maintenance of ColE1-related plasmids, was compared with the PIR sequence database. Strong local similarities to the sequence of the E. coli protein tRNA nucleotidyltransferase were found. Since a substrate of the latter protein, tRNA, structurally resembles the RNAs that control ColE1 copy number we believe that we may have identified a region in PcnB that interacts with these RNAs. Consistent with this idea is our observation that PcnB is required for the replication of R1, a plasmid whose replication is also regulated by a small RNA. |
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Keywords: | Plasmid copy number tRNA nucleotidyltransferase PcnB Replication control ColE1 |
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