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Barley protein function as growth inhibitor in Saccharomyces cerevisiae
Authors:Xiaolei Jiang  Xueyan Dong  Jun Yao  Liang Dong  Xia Chen  Mingda Li  Changxin Zhao
Affiliation:1. Liaoning Key Laboratory of Fermentation Technology, School of Biological Engineering, Dalian Polytechnic University, Qinggongyuan Road No.1, Dalian, 116034, China
Abstract:The extracted toxic barley protein showed different protein content depending on the barley strain and demonstrated an inhibitory effect on the growth of yeast and glucose consumption. Chinese barley Danpi-2 had the highest concentration of toxic protein of all the types barley tested. When the concentration of proteins was increased to 32 μg/ml, the inhibition ratio also went up by 50%. The SDS-PAGE electrophoresis pattern showed that the protein was stable and tolerant at the temperature of 100°C.
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