Barley protein function as growth inhibitor in Saccharomyces cerevisiae |
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Authors: | Xiaolei Jiang Xueyan Dong Jun Yao Liang Dong Xia Chen Mingda Li Changxin Zhao |
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Affiliation: | 1. Liaoning Key Laboratory of Fermentation Technology, School of Biological Engineering, Dalian Polytechnic University, Qinggongyuan Road No.1, Dalian, 116034, China
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Abstract: | The extracted toxic barley protein showed different protein content depending on the barley strain and demonstrated an inhibitory effect on the growth of yeast and glucose consumption. Chinese barley Danpi-2 had the highest concentration of toxic protein of all the types barley tested. When the concentration of proteins was increased to 32 μg/ml, the inhibition ratio also went up by 50%. The SDS-PAGE electrophoresis pattern showed that the protein was stable and tolerant at the temperature of 100°C. |
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