Functional screening for salinity tolerant genes from <Emphasis Type="Italic">Acanthus ebracteatus</Emphasis> Vahl using <Emphasis Type="Italic">Escherichia coli</Emphasis> as a host |
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| Authors: | P D Nguyen C-L Ho J A Harikrishna M C V-L Wong R Abdul Rahim |
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| Institution: | (1) Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;(2) Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;(3) Biotechnology Research Institute, Universiti Malaysia Sabah, Locked Bag 2073, 88999 Kota Kinabalu, Sabah, Malaysia;(4) Department Bioprocess, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;(5) College of Agriculture and Applied Biology, CanTho Univerisity, 3-2 street, CanTho City, Vietnam |
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| Abstract: | Salinity reduces plant growth and crop production globally. The discovery of genes in salinity tolerant plants will provide
the basis for effective genetic engineering strategies, leading to greater stress tolerance in economically important crops.
In this study, we have identified and isolated 107 salinity tolerant candidate genes from a mangrove plant, Acanthus ebracteatus Vahl by using bacterial functional assay. Sequence analysis of these putative salinity tolerant cDNA candidates revealed that 65% of them have not been reported to
be stress related and may have great potential for the elucidation of unique salinity tolerant mechanisms in mangrove. Among
the genes identified were also genes that had previously been linked to stress response including salinity tolerance, verifying
the reliability of this method in isolating salinity tolerant genes by using E. coli as a host. |
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| Keywords: | Acanthus ebracteatus Functional screening Mangrove Salinity tolerance |
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