Acrylamide inhibits gravitropism and affects microtubules in rice coleoptiles |
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Authors: | C Gutjahr P Nick |
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Institution: | Institut für Biologie II, Albert-Ludwigs-Universit?t Freiburg, Freiburg im Breisgau Botanisches Institut 1, Universit?t Karlsruhe, Karlsruhe
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Abstract: | Summary. To find components which participate in gravitropic signal transmission, we screened different cell biological inhibitors
for their effect on gravitropic bending of rice coleoptiles. Acrylamide, which is known to affect intermediate filaments in
mammalian cells, strongly inhibited gravitropic bending at concentrations that did not inhibit growth of coleoptile segments.
This inhibition was reversible. Investigating the acrylamide effect further, we found that it interferes with an event that
occurs around 15 min after the onset of stimulation. We also observed that acrylamide inhibits polar indolyl-3-acetic acid
transport. Furthermore, acrylamide efficiently eliminated microtubules, whereas actin filaments remained intact. To our knowledge
this is the first report of effects of monoacrylamide in plant cells.
Correspondence and reprints (present address): Laboratoire de Génétique Végétale, Sciences III, Université de Genève, 30 Quai
Ernest-Ansermet, 1211 Genève, Switzerland. |
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Keywords: | : Acrylamide Auxin transport Gravitropism Microtubule Oryza sativa L Rice |
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