| 克隆差异表达基因的新策略 |
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| 引用本文： | 崔大祥,闫小君,王枫,苏成芝.克隆差异表达基因的新策略[J].生物化学与生物物理进展,2000,27(4):362-364. |
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| 作者姓名： | 崔大祥 闫小君 王枫 苏成芝 |
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| 作者单位： | 第四军医大学全军基因诊断技术研究所,西安,710032 |
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| 摘 要： | 基因表达的变化有两种，即新出现的基因表达与表达量差异的基因表达.表达量差异的基因克隆技术主要有mRNA差异展示，此技术是目前筛选差异表达基因最有效的方法之一，但主要存在假阳性率高的不足，针对此缺点，近几年提出了新的策略与方法，如差异消减展示、基于PCR和减法杂交基础上的差异表达基因克隆技术，这些技术具有显著优势.
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| 关 键 词： | 基因表达，克隆，策略 |
| 收稿时间： | 1999/6/28 0:00:00 |
| 修稿时间： | : |
New Strategy of Cloning of Differentially Expressed Genes |
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| CUI Da-Xiang,YAN Xiao-Jun,WANG Feng and SU Cheng-Zhi.New Strategy of Cloning of Differentially Expressed Genes[J].Progress In Biochemistry and Biophysics,2000,27(4):362-364. |
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| Authors: | CUI Da-Xiang YAN Xiao-Jun WANG Feng and SU Cheng-Zhi |
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| Abstract: | There are two kinds of changes of gene expression,that is,novel gene expression and differential gene expression in quantity. Cloning technique of differentially expressed gene in quantity mainly is mRNA differential display, which is presently one of the most effective methods. However, mRNA differential display possesses higher unreal positive rate,in order to overcome its shortcoming, some novel strategies and methods were advocated,such as differential subtraction display, subtraction based on LD-PCR, LD-PCR based on subtraction,those techniques have dominant advantages to mRNA differential display. |
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| Keywords: | gene expression cloning strategy |
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