| 编码酵母丙氨酸tRNA两个半分子的DNA的克隆 |
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| 引用本文： | 刘建华,王德宝.编码酵母丙氨酸tRNA两个半分子的DNA的克隆[J].中国生物化学与分子生物学报,1992,8(4):488-491. |
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| 作者姓名： | 刘建华 王德宝 |
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| 作者单位： | 中国科学院上海生物化学研究所分子生物学国家重点实验室，中国科学院上海生物化学研究所分子生物学国家重点实验室 上海 200031，上海 200031 |
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| 摘 要： | 用DNA合成仪合成寡聚脱氧核苷酸。用T4-DNA连接酶把这些寡聚脱氧核苷酸重组成双链DNA。这两个双链DNA的上游是T7-启动子,下游分别编码酵母丙氨酸tRNA的5′半分子(1-35位核苷酸)和3′半分子(35-76位核苷酸)。再把这两个双链DNA克隆到PUC 12质粒中。经点杂交筛选和DNA顺序测定证明克隆是成功的。
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| 关 键 词： | DNA化学合成 DNA克隆 酵母丙氨酸tRNA |
| 收稿时间： | 1992-08-20 |
Cloning of DNAs Coding for the Two Half Molecules of Yeast Alanine tRNA |
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| Liu,Jian-hua Wang,De-bao.Cloning of DNAs Coding for the Two Half Molecules of Yeast Alanine tRNA[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(4):488-491. |
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| Authors: | Liu Jian-hua Wang De-bao |
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| Institution: | (T.P.Wang)(Shanghai Molecular Biology Laboratory,Shanghai Institute of Biochemistry,Academia Sinica 200031 |
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| Abstract: | Six oligodeoxynucleotides were synthesized on a DNA synthesizer.These fragments were constructed into two DNA double strands with T4-DNA ligase.The upstream sequences of both double strands were T7-promoter, and their downstream sequences were coding for the 5'-half molecule (1-35 nucleotides) and the 3'-half molecule (35-76 nucleotides) of yeast alanine tRNA repectively.These double strands were cloned into a plamid PUC 12.The successful cloning was confirmed by site hybrid screening and DNA sequencing. |
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| Keywords: | Chemical synthesis of DNA DNA cloning Yeast alanine tRNA |
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