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A rapid and high yielding DNA miniprep for cotton (Gossypium spp.)
Authors:Haiwen Li  Jinhua Luo  John K Hemphill  Jau-Tay Wang  Jean H Gould
Institution:(1) Department of Forest Science, Texas A&M University, 77843-2135 College Station, Texas, USA;(2) AgriVitis, Inc., 2191 San Juan-Hollister Road, 95045 San Juan Bautista, CA, USA;(3) 4 Lane 9 Minsheng Rd., Wufeng 413, Taichung, Taiwan
Abstract:A rapid DNA minipreparation method was developed for cotton and yields 500–600 μg DNA from 1.0 g fresh leaf tissue. Cotton DNA extracted using this method is completely digested with restriction enzymes, supports PCR and Southern DNA analyses and was used successfully in these applications. An erratum to this article is available at .
Keywords:DNA extraction            Gossypium            minipreparation
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