Effects of melanin on high- and low- linear energy transfer (LET) radiation response of human epithelial cells |
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Authors: | G F Grossi M Durante G Gialanella M Pugliese I Mosse |
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Institution: | (1) Dipartimento di Scienze Fisiche, Università di Napoli “Federico II” and Istituto Nazionale di Fisica Nucleare, Sezione di Napoli, Mostra d'Oltremare Padiglione 20, I-80125 Naples, Italy, IT;(2) Servizio di Radioprotezione, Università di Napoli “Federico II”, Mostra d'Oltremare Padiglione 20, I-80125 Naples, Italy, IT;(3) Institute of Genetics and Cytology of Belarus Academy of Sciences, 220072 Minsk, Belarus, |
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Abstract: | The search for effective radioprotectors is of major concern in the medical, military, environmental, and space sciences.
Conventional radioprotectors are generally effective only during a single irradiation and display their radioprotective properties
only at high, toxic concentrations. In addition, they reduce somatic radiation effects but are poorly efficient in protecting
from hereditary stochastic radiation effects. In this respect, the pigment melanin merits attention. Experiments referring
to potential melanin effects on the ionising radiation response have been carried out with different biological systems, both
in vivo and in vitro. In this paper, we present results on the response to high- and low-linear energy transfer (LET) radiation
of a human mammary epithelial cell line, H184B5 F5-1 M/10, supplemented by melanin. The incorporation of auto-oxidative (l-dopa) melanin was linear for concentrations from 3 to 10 μg/ml in the growth medium. Concentrations of up to 250 μg/ml did
not significantly impair the cells proliferative ability. No significant protective effect of melanin on the survival of cultured
cells after exposure to alpha-particles (130 keV/μm) or x-rays was observed.
Received: 24 March 1997 / Accepted in revised form: 14 November 1997 |
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