Modelling of oligosaccharide synthesis by dextransucrase |
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Authors: | Demuth B Jördening H J Buchholz K |
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Affiliation: | Lehrstuhl für Technologie der Kohlenhydrate, Technical University of Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany. |
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Abstract: | Dextransucrase catalyses the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. Much experimental work has been carried out and a scheme of reactions and a mathematical model have been developed to describe the complex kinetic behaviour of the enzyme. A computer program was used to calculate the parameters of the model from a broad range of experimental data, investigating a large number of kinetic tests with the acceptors maltose and fructose. The results lead to design considerations for a continuous reactor system with immobilized dextransucrase to produce leucrose, a disaccharide of industrial interest. |
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