Improved guggulsterone production from sugars, precursors, and morphactin in cell cultures of Commiphora wightii grown in shake flasks and a bioreactor |
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Authors: | Meeta Mathur K G Ramawat |
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Institution: | (1) Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, 313001, India |
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Abstract: | Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to 18 μg
l−1 was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine,
pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of 10 g l−1 biomass and ∼200 μg l−1 guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and 67 μg l−1 guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation.
2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii. |
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Keywords: | Bioreactor Commiphora wightii Guggulsterone Morphactin Secondary metabolites |
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