急性高原肺水肿患者血清中VEGF,TNF-α,IL-6及NO的含量变化

目的：检测高原肺水肿患者发生及转归过程中内皮生长因子（VEGF）,肿瘤坏死因子-α（TNF-α）,白介素-6（IL-6）和一氧化氮（NO）的含量变化,探讨高原肺水肿发生发展可能的病理生理机制。方法：收集10例高原肺水肿患者治疗前和转归后的血清样本,采用酶联免疫法和硝酸还原测定VEGF,TNF-α,IL-6和NO的含量。结果：VEGF在高原肺水肿发病时血清的含量为（167.9±26.5）pg/ml,而转归之后其含量显著降低为（53.1±17.0）pg/ml,（P〈0.01）;TNF-α在血清中的含量从发病时的（86.2±24.1）pg/ml减少到转归后的（29.2±6.8）pg/ml,（P〈0.05）,有显著性差异。而IL-6的水平也从发病时的（32.3±16.5）pg/ml变化为转归后的（12.5±8.0）pg/ml,虽然有下降的趋势,但没有统计学差异。而高原肺水肿患者血清中的NO水平从发病时的（33.8±3.3）μmol/L明显的升高到转归后的（74.1±6.2）μmol/L,（P〈0.01）。结论：VEGF,TNF-α,IL-6和NO参与了高原肺水肿的发生和转归过程。

Changes of VEGF, TNF-alpha, IL-6 and NO in serum of patients with HAPE

Objective：To explore the possible pathophysiological process and mechanisms underlying the development and formation of high altitude pulmonary edema（HAPE） by observing the changes in contents of VEGF,TNF-α,IL-6 and NO in serum from the initiated and recovery of HAPE patients.Methods：We studied 10 HAPE patients in a Chinese population.The patients were divided into two groups including HAPE initiate group and the recovery group.Contents of VEGF,TNF-α,IL-6 and NO in serum of the two groups were determined to study the process of HAPE.Results：VEGF levels in the HAPE initiate one and the recovery groups were（167.9±26.5 and 53.1±17.0 pg/ml）,respectively.There was a significant decrease of VEGF content in recovery one compared to the HAPE group.The same results for TNF-α were gained.The levels of TNF-α in recovery group was much lower than that in the HAPE initiate one.They were（29.2±6.8）pg/ml and（86.2±24.1）pg/ml,respectively.The contents of IL-6 in HAPE initiate group and the recovery group were（32.3±16.5）pg/ml and（12.5±8.0）pg/ml,respectively.But no significance existed.The level of NO in HAPE initiate group was（33.8±3.3）μmol/L,and it remarkably increased to（74.1±6.2）μmol/L in recovery one.Conclusion：VEGF,TNF-α,IL-6 and NO participated in the different aspects of the pathophysiological process and might have influence on HAPE.