Immunogenicity and toxicity of lipopolysaccharide from Francisella tularensis LVS |
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| Authors: | G Sandström A Sjöstedt T Johansson K Kuoppa JC Williams |
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| Institution: | Division of Microbiology, National Defence Research Establishment, Umeå, Sweden; U.S. Army Medical Research Institute of Infectious Diseases, Intracellular Pathogens Branch, Fort Detrick, Frederick, Maryland, USA; Department of Infections Diseases, University of Umeå, Umeå, Sweden; Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA |
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| Abstract: | Abstract Lipopolysaccharide (LPS) from the live vaccine strain of Francisella tularensis ( F . tularensis LVS) was isolated and purified. The LPS did not stimulate lymphocytes from previously tularaemia-vaccinated individuals or lymphocytes from nonprimed individuals. However, serum antibodies from tularaemia vaccines reacted with the LPS whereas virtually no reactivity was found with antibodies from individuals not exposed to F. tularensis LVS. Antibodies of immunoglobulin class M displayed the antibody reactivity predominantly. The LPS failed to induce the mononuclear cell-derived cytokine interleukin-1 and only low levels of tumour necrosis factor were detected. Furthermore, no LPS endotoxin properties were found in galactosamine-treated mice or in the Limulus amoebocyte lysate assay. From these results it can be concluded that F. tularensis LVS possesses a lipopolysaccharide-like molecule, which does not exhibit properties of a classical endotoxin. |
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| Keywords: | Lipopolysaccharide Tularaemia Francisella tularensis Endotoxin TNF IL-1 |
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