Cyclic AMP modulates insulin binding and induces post-receptor insulin resistance of glucose transport in isolated rat adipocytes |
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Authors: | Diethelm Kirsch W Kemmler HU Häring |
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Institution: | 1. Institut für Diabetesforschung, Kölner Platz 1, 8000 München 40, FRG;2. Joslin Research Laboratory, Harvard Medical School, Boston, Massachusetts 02215, USA |
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Abstract: | The effect of cAMP on insulin binding and insulin stimulation of glucose transport was investigated in isolated rat adipocytes. Preincubation for 30 min in medium containing 16 mmol/l glucose and either db-cAMP or bromo-cAMP in concentrations of 10(-4)-10(-3) M inhibited high affinity binding of insulin by 15 to 30% and glucose transport by 30 to 50%. Preincubation with IBMX (10(-4)-10(-3) M) reduced insulin binding by 25% and glucose transport by 70%. Closer analysis of these data indicated that preincubation with these compounds caused not only a decrease in insulin binding but also a post-receptor resistance. High intracellular cyclic AMP-levels seem therefore to induce insulin resistance at both receptor and post-receptor levels. |
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Keywords: | cAMP adenosine cyclic 3′ 5′ monophosphate IBMX 3-isobutyl-1-methylxanthine db-cAMP |
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