Experience in using an ethanol sensor to control molasses feed-rates in baker's yeast production |
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Authors: | J P Axelsson C F Mandenius O Holst P Hagander B Mattiasson |
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Institution: | (1) Department of Automatic Control, Lund Institute of Technology, P.O. Box 118, S-22100 Lund, Sweden;(2) Department of Pure and Applied Biochemistry, Chemical Center, Lund Institute of Technology, P.O. Box 118, S-22100 Lund, Sweden;(3) Department of Biotechnology, Chemical Center, Lund Institute of Technology, P.O. Box 118, S-22100 Lund, Sweden |
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Abstract: | An ethanol sensor has been tested for feed-rate control of baker's yeast prouction. The yeast was grown on molasses in an 8 dm3 fed-batch reactor up to a cell concentration of 60–70 kg/m3. Studies were made on three levels: reliability of the sensor system, characterisation of the control problem, and evaluation of ethanol-controlled cultivations in terms of yield and production rate. Arguments are given for the conceptual advantages of ethanol control compared to other methods of substrate control. It is also shown that ethanol control allows for a simple regulator structure. In fact, a PID regulator, with constant parameters, was used around an exponential dosage scheme. Tuning of the regulator parameters was performed by using simulation on a simplified model of the process. Several cultivations have been carried out. Results from four comparable cultivations are given in detail, and the experience from many others is summarized. |
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