A stably transfected c-myc cat hybrid gene is not regulated by serum in NIH3T3 cells期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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A stably transfected c-myc cat hybrid gene is not regulated by serum in NIH3T3 cells

Authors:	M G?tschl D Eick

Institution:	1. Department of Neurology, Heimer Institute for Muscle Research, BG-University Hospital Bergmannsheil, Ruhr-University Bochum, 44789 Bochum, Germany;2. Department of Human Genetics, Ruhr-University Bochum, 44801 Bochum, Germany;3. Department of Cytology, Ruhr-University Bochum, 44801 Bochum, Germany;4. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr-University Bochum, 44801 Bochum, Germany;1. Department of Neurology, Heimer Institute for Muscle Research, BG-University Hospital Bergmannsheil, Ruhr-University Bochum, 44789 Bochum, Germany;2. Genetikum, Center for Human Genetics, 89231 Neu-Ulm, Germany;3. Department of Human Genetics, Ruhr-University Bochum, 44801 Bochum, Germany;4. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr-University Bochum, 44801 Bochum, Germany

Abstract:	A plasmid containing the CAT (chloramphenicolacetyltransferase) gene fused to the 5' adjacent sequences and first exon of the human c-myc gene was stably transfected into NIH3T3 cells. Single cell clones were grown and CAT activity was measured after serum starvation and stimulation. CAT activity of the hybrid construct remained unchanged in serum-deprived and serum-stimulated cells. In contrast the steady-state RNA level of the endogenous mouse c-myc gene was strongly elevated upon serum stimulation. The bona fide usage of the human c-myc promoter P1/P2 in mouse cells carrying the hybrid gene was revealed by S1 analysis.

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