cGMP对原代培养猪冠状动脉平滑肌细胞钙激活钾通道的作用

３′,５′－环－磷酸鸟苷（ｃＧＭＰ）具有激活血管平滑肌细胞膜上钙激活钾通道（ＫＣａ通道）的作用,从而引起血管平滑肌细胞的舒张。但ｃＧＭＰ激活ＫＣａ物机制存在争论。本工作应用膜片箝技术以原代培养猪冠状动脉平滑肌细胞为对象研究了ｃＧＭＰ影响ＫＣａ通道的机制。实验结果显示：（１）在ｃｅｌｌ－ａｔｔａｃｈｅｄ膜片方式下,当溶液内游离Ｃａ＾２＋浓度为１０＾－７ｍｏｌ／Ｌ,膜电位为＋７０ｍＶ时,不同浓度的ｃＧ

Effect of cGMP on calcium-activated potassium channels in primary cultured porcine coronary artery smooth muscle cells

Guanosine 3',5'-cyclic monophosphate (cGMP) is capable of relaxing vascular smooth muscle through activating calcium-activated potassium channels (KCa channel) in the vascular smooth muscle cell membrane. But regarding the mechanism it is still under debate. In the present work the mechanism of the effect of cGMP on KCa channel in primary cultured porcine coronary artery smooth muscle cells using patch clamp technique was investigated. Experimental results showed that (1) different concentrations of 8-bromo-cGMP (0.25 mmol/L, 0.50 mmol/L, 1.00 mmol/L), serving as a membrane permeable analogue, could activate KCa channels, and (2) 1.00 mmol/L 8-bromo-cGMP could not activate such kind of channels in inside-out patch under the same condition. These results suggested that the activating effect of cGMP on KCa channels was indirect, being mediated by some intracellular process.