Fetal-Type Phosphorylation of the τ in Paired Helical Filaments |
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Authors: | Kazutomi Kanemaru Koji Takio† Reiko Miura Koiti Titani Yasuo Ihara‡ |
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Institution: | Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, University of Tokyo, Japan. |
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Abstract: | To determine the phosphorylation sites of the tau in paired helical filaments (PHF), two types of PHF antisera with different specificities were used: One was a conventional anti-PHF, and the other was an antiserum to formic acid-denatured PHF (anti-HFoPHF). Phosphorylated tau-specific antibodies, anti-ptau 1 and anti-ptau 2, were prepared from anti-PHF and anti-HFoPHF, respectively. We found that both anti-ptau 1 and anti-ptau 2 labeled fetal or juvenile tau but not adult tau. The anti-ptau 1- and anti-ptau 2-recognition sites were immunochemically localized to the fragment Asp313 to Ile328 in the most COOH-terminal portion of tau. Furthermore, Ser315 was determined as the anti-ptau 2 recognition site. The sequence surrounding Ser315 was not found in the canonical sequences phosphorylated with known kinases. |
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Keywords: | Alzheimer's disease Paired helical filaments Phosphorylation Anti-paired helical filaments Immunochemical approach |
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