| 马动脉炎病毒核蛋白基因的克隆与表达 |
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| 引用本文： | 杜建 王志亮 赵永刚 宋厚辉 金宁一 张念祖. 马动脉炎病毒核蛋白基因的克隆与表达[J]. Virologica Sinica, 2005, 20(3): 323-325 |
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| 作者姓名： | 杜建 王志亮 赵永刚 宋厚辉 金宁一 张念祖 |
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| 作者单位： | 农业部动物检疫所国家外来动物疫病诊断中心，农业部动物检疫所国家外来动物疫病诊断中心，农业部动物检疫所国家外来动物疫病诊断中心，中国科学院微生物研究所，解放军军需大学病毒基因工程重点实验室，农业部热带亚热带动物病毒学重点实验室 山东青岛266032 昆明市畜牧兽医站，云南昆明650223，山东青岛266032，山东青岛266032，北京100088，吉林长春130062，云南昆明650224 |
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| 基金项目： | 农业部诊断技术标准和农业部热带亚热带动物病毒学重点实验室开放基金(200205) |
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| 摘 要： | 马病毒性动脉炎是危害世界养马业的重要传染病之一,是由动脉炎病毒科动脉炎病毒属的马动脉炎病毒(Equinearteritisvirus,EAV)引起的一种以病马发热,步态僵直,躯干及眼周围水肿,并出现粘液脓性鼻炎、结膜炎,外生殖道水肿为特征的传染病,对妊马能引起流产,使易感怀孕母马的流产率
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| 关 键 词： | 马动脉炎病毒 核衣壳蛋白 表达 纯化 |
Cloning and Expression of Nucleocapsid Protein Gene of Equine Arteritis Virus in E.coli |
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| DU Jian {,},WANG Zhi-liang ,ZHAO Yong-gang ,SONG Hou-hui ,JIN Nin-Yi ,ZHANG Nian-zu. Cloning and Expression of Nucleocapsid Protein Gene of Equine Arteritis Virus in E.coli[J]. 中国病毒学(英文版), 2005, 20(3): 323-325 |
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| Authors: | DU Jian { } WANG Zhi-liang ZHAO Yong-gang SONG Hou-hui JIN Nin-Yi ZHANG Nian-zu |
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| Affiliation: | DU Jian {1,3},WANG Zhi-liang 1,ZHAO Yong-gang 1,SONG Hou-hui 4,JIN Nin-Yi 5,ZHANG Nian-zu 2 ** |
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| Abstract: | The gene of nucleocapsid protein of Equine arteritis virus was amplified from PMD-18-T plasmid with equine arteritis virus ORF7 sequence by PCR. The PCR product was sequenced as well as purified and digested with EcoR I and Xho I, then directly cloned into the prokaryotic vector pET32a. Consequently the recombinant plasmid was constructed, designateds pET32a-N. PET32a-N was transformed into the host cell BL21(DE3) and the expression procedure was optimized including cultivated temperature, optional induction concentration and time of IPTG. The result indicated that the nucleocapsid protein can be expressed efficiently with 0.8mmol/L IPTG and 4 hour induction. The resulting Trx-N recombinant fusion protein was identified to be consisted of 34 kDa protein by SDS-PAGE and western blotting analysis. It indicated that the recombinant fusion protein could be used as antigen of diagnostic assay for detecting antibodies. |
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| Keywords: | Equine arteritis virus Neucleocapsid protein Cloning Expression |
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