Different forms of the cobalt-bleomycin A2 complex |
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Authors: | Cornelis M Vos Gerrit Westera Benjamin van Zanten |
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Institution: | 1. Radionuclidencentrum. Vrije Universiteit. c/o de Boelelaan 1083a. Amsterdam. The Netherlands;2. Faculty of Medicine. Vrije Universiteit. c/o de Boelelaan 1083a. Amsterdam. The Netherlands |
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Abstract: | Chromatographic analysis of Co-bleomycin on CM Sephadex C25 or silica shows that known components like bleomycin A2, B2, and A1 can be separated into two forms. We observed an equilibrium between both forms and saw that the addition of certain salts influences the transition of one form into the other. A reaction scheme is suggested in which a different occupation of the sixth binding site of cobalt is correlated with the different forms observed. These different complexes may be an intramolecular adduct of the carbamoyl moiety of bleomycin, an external carboxylate adduct or an oxygen or hydroxyl adduct. |
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Keywords: | blm bleomycin TLC thin layer chromatography LC ion exchange liquid chromatography HPLC high-performance liquid chromatography UV ultraviolet RA radio activity |
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