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Kinetic analysis of calcium binding to concanavalin A
Authors:Stephen D. Stroupe  Ronald J. Doyle
Affiliation:Department of Biochemistry and Department of Microbiology and Immunology, University of Louisville, KY, USA
Abstract:The kinetics of calcium binding to concanavalin A was studied utilizing ultraviolet difference spectral measurements. The results show that calcium binds to the lectin in a biphasic process: a rapid and reversible phase, followed by a relaxation phase with a kobs of 0.012 sec?1. Kinetic measurements were used to calculate the association constant, Ka, for calcium binding to concanavalin A of 2.7 x 104 M?1, in reasonable agreement with values obtained by equilibrium methods.
Keywords:Address reprint requests to: Ronald J. Doyle   Department of Microbiology and Immunology   University of Louisville   Schools of Medicine and Dentistry   Louisville   KY 40232   USA
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