Inflammatory serum proteome pattern in mice fed a high-fat diet |
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Authors: | Elisabetta Gianazza Cristina Sensi Ivano Eberini Federica Gilardi Marco Giudici Maurizio Crestani |
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Affiliation: | 1. Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133, Milan, Italy 2. Laboratorio “Giovanni Galli” di Biochimica e Biologia Molecolare del Metabolismo e Spettrometria di Massa, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133, Milan, Italy
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Abstract: | To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins, haptoglobin and hemopexin, were significantly up-regulated in animals receiving the high-fat diet. Data on all other proteins also pointed to an inflammatory condition in these animals. The largest change in concentration was observed for carboxylesterase N, a circulating enzyme seldom connected with lipid metabolism in earlier reports. These observations agree with the notion of a link between diet-induced hyperlipidemia and the inflammatory component of its cardiovascular sequels in humans, but the effects in the experimental animals are massive and obviously affect most of the major serum proteins. |
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