Comparative characterization of growth and recombinant protein production among three insect cell lines with four kinds of serum free media |
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| Authors: | Mi?Sun?Kwon Takashi?Dojima Email author" target="_blank">Enoch?Y?ParkEmail author |
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| Institution: | (1) Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529 Shizuoka, Japan;(2) Production Technology Laboratory, Kaken Pharmaceutical Co. Ltd., 301 Gensuke, 426-8646 Fujieda, Japan |
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| Abstract: | Three insect cell lines, Sf9, Sf21 and Tn5B1-4, and four different kinds of serum free media (SFM), Sf 900 II, EX-CELL 420,
EX-CELL 405 and Express Five, were used to compare the nutrient consumption, byproduct formation, production of recombinant
protein and protease activity in suspension cultures. The Sf 900 II SFM was appropriate for the cell growth and protein production
of the Sf9 and Sf21 cell lines. When the Tn5B1-4 cell line was grown in the Express Five SFM, the specific growth rate was
1.6 fold higher than those of either the Sf9 or Sf21 cell lines. The glucose and glutamine consumption rates per cells, were
4 and 2.3. times higher than those of the Sf9 cell line, respectively. The overall yield coefficients of the lactate and ammonium
ion were 2.8 and 1.5 times higher compared to those of the Sf9 cell line, respectively. The maximum specific β-galactosidase
production rate was 4.5. fold that of the Sf9 cell line, a 3 times higher protease activity per cell. |
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| Keywords: | baculovirus expression vector system serum free medium insect cell β -galactosidase protease |
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