Immunocytochemical localization of oviduct-specific glycoproteins in the oviductal epithelium from cows at follicular and luteal phases |
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Authors: | Hiroyuki Abe Chikako Numazawa Miki Abe Masakazu Onodera Akira Katsumi |
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Affiliation: | (1) Department of Morphology, Institute of Endocrinology, Gunma University, 3-39-15, Showa-machi, 371 Maebashi-shi, Gunma-ken, Japan;(2) Present address: Groupe de Neuroendocrinologie Cellulaire et Molèculaire, 11, Collège de France, CNRS, Place Marcelin Berthelot, F-75231 Paris Cedex 05, France |
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Abstract: | An immunoelectron-microscopic and morphometric study was carried out on the anterior pituitary prolactin (PRL) cells of adult male Wistar rats treated with a combination of thyroidectomy and administration of L-thyroxine (T4) and/or synthetic thyrotropin-releasing hormone (TRH) in order to clarify the effects of changes in the hypothalamus-pituitary-thyroid axis on the ultrastructure and function of PRL cells. After thyroidectomy, PRL cells underwent atrophy and hypofunction of their cell organelles, but these changes tended to be restored to their normal level by T4 treatment. On the other hand, the administration of TRH to intact rats produced hypertrophy and hyperfunction in the PRL cells, although this treatment had no effect on the PRL cells of thyroidectomized rats. However, treatment with a combination of T4 and TRH had a strong effect and led to hypertrophy and hyperfunction in the PRL cells of thyroidectomized rats. Serum and pituitary PRL levels were measured by radioimmunoassay (RIA) for a comparison with the morphological results. They correlated well with the morphological changes. These results indicate that TRH stimulates PRL secretion in the presence of thyroid hormone, and that the thyroid hormone plays an important role in the basic maintenance of PRL cell function and its reactivity to TRH. |
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Keywords: | Anterior pituitary Prolactin cell TRH T4 Thyroidectomy Immuno-electron microscopy Morphometry Rat (Wistar) |
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