Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level |
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| Authors: | M Thiry D Dombrowicz |
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| Institution: | Laboratoire de Cytologie et Histologie animale, Liege, Belgique. |
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| Abstract: | A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled. |
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| Keywords: | DNA detection DNA immunolocalization Chromatin Monoclonal antibody |
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