Separation of Cl-Containing Chlorophylls by Column Chromatography |
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Authors: | Katoh, Tetzuya Yasuda, Kyoko |
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Affiliation: | Department of Botany, Faculty of Science, Kyoto University Kita-shirakawa, Kyoto 606, Japan |
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Abstract: | Using Toyopearl and cyclohexane: cyclohexanol solvent, fourCl-containing Chls were separated from 36Cl-labeled cells ofthe blue-green, Plectonema boryanum. In normally grown cells,all four Cl-containing chlorophylls amounted to less than 1/2,000of the total Chi and about 1/50 of P700, values much lower thanpreviously reportedcontents of Chi RC I, and varied from algato alga. The level of Cl-containing Chi was markedly enhancedwhen the cells were poisoned with methyl viologen. These resultssuggests that these Cl-containing Chls are not related to thereaction center of PS I. (Received June 23, 1987; Accepted September 17, 1987) |
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