| 休哈塔假丝酵母（Candida shehatae）木糖醇脱氢酶基因（xyl2）克隆与序列分析 |
| |
| 引用本文： | 洛雪,葛菁萍,平文祥. 休哈塔假丝酵母（Candida shehatae）木糖醇脱氢酶基因（xyl2）克隆与序列分析[J]. 生物信息学, 2009, 7(3): 240-242 |
| |
| 作者姓名： | 洛雪 葛菁萍 平文祥 |
| |
| 作者单位： | 微生物黑龙江省高校重点实验室,黑龙江大学生命科学学院,哈尔滨,150080 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)子课题--木质纤维素原料生物高效转化技术及产品;子课题 |
| |
| 摘 要： | 根据木糖醇脱氢酶基因序列相似的特点，根据树干毕赤酵母（Pichia．sfipitis），热带假丝酵母（Candidatropicalis）设计1对简并引物获得C．shehatae HDYXHT－01木糖醇脱氢酶基因的序列，此片段长为1095bp。利用生物信息学软件对此序列进行了同源性分析、氨基酸组成分析、疏水性分析、磷酸化位点预测、CDS分析、二级结构预测。结果表明，该克隆片段为C．shehataeHDYXHT－01木糖醇脱氢酶基因的序列。
|
| 关 键 词： | 休哈塔假丝酵母 木糖醇脱氢酶基因 序列分祈 结构预测 |
Cloning and Sequence Analysis of the xylitol dehydrogenase Gene (xyl2)from Candida shehatae |
| |
| LUO Xue,GE Jing-ping,PING Wen-xiang. Cloning and Sequence Analysis of the xylitol dehydrogenase Gene (xyl2)from Candida shehatae[J]. Chinese Journal of Bioinformatics, 2009, 7(3): 240-242 |
| |
| Authors: | LUO Xue GE Jing-ping PING Wen-xiang |
| |
| Affiliation: | ( Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China) |
| |
| Abstract: | One primer is designed to obtain the xylitol dehydrogenase gene conserved sequence motifs in C.shehatoe HDYXHT- 01, which has the similar structure of Pichia. stipitis and Candida tropicalis,This amplified fragment is about 1 095 bp. Ihe sequence was analyzed by the following methods, the homology sequence analysis, amino acid composition, hydrophobicity analysis, predicition of phosphoralation sites, conserved domains analysis and secondary stnlcture. The restdts show that the fragment is xylitol dehydrogenase gene in C. shehatoe HDYXHT - 01. |
| |
| Keywords: | C. shehatae HDYXHT-01 xyhtol dehydrogenase gene sequence analysis structure prediction |
| 本文献已被 维普 万方数据 等数据库收录！ |
